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Many studies have addressed the hazardous role of cigarette smoking on male fertility, but the exact molecular mechanisms involved in the impairments caused by nicotine remain unclear. To evaluate the detrimental effects of nicotine exposure on spermatogenesis, two‐dimensional gel electrophoresis and mass spectrometry analysis were performed to screen and identify differentially expressed proteins...
Little is known about the effects of brief potassium depolarization that occurs concurrently with transient ischemia, epilepsy and head trauma. To investigate the effect of short-term depolarization on light (NF-L), middle (NF-M), and heavy (NF-H) neurofilament proteins and determine the role played by calcium in that effect, mixed septo-hippocampal cultures were exposed to 60 mM K + for...
In septo-hippocampal cell cultures, brief potassium depolarization produces calcium-dependent decreases in neurofilament proteins and loss of fine neuritic processes within 24 h. It is not known whether neurons later exhibit delayed degeneration and die, live with enduring neurofilament loss, or restore neurofilament protein levels. Therefore, we exposed septohippocampal cultures to 6 min potassium...
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