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A general method was developed for the synthesis of serine or threonine containing cyclic peptides utilizing the β-hydroxyl side-chain of these residues as an anchor point to Wang resin. The peptide chain was assembled by conventional Fmoc/tBu solid-phase chemistry followed by palladium catalyzed exposure of the allyl protected C-terminus group and on-resin cyclization. The cyclic heptapeptide stylostatin...
A phase sensitive 3D J-resolved HMBC experiment has been developed to obtain well-separated multiple-bond and one-bond heteronuclear correlation spectra. This experiment greatly facilitates spectral assignment and accurate measurement of the long-range 1 H- 13 C J coupling constants by a least-squares method.
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