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Ligands containing acetamido group and a spacer were conjugated with an acrylic monomer and copolymerized with N-isopropylacrylamide (NIPAM) to yield a thermoprecipitating polymer. The ability of the ligand to bind to lysozyme, which is the first step in the separation of lysozyme, is quantified in terms of I 50 , the ligand concentration required to achieve 50% of the maximum attainable...
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