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Reactive oxygen intermediates have been implicated in the transduction of TNFα signals, although the source of such oxidants has not been established. We found that activation of ECV-304 cells by TNFα was accompanied by a transient burst of oxidants and activation of JNK, both of which were suppressed by two distinct inhibitors of the phagocyte NADPH oxidase and the thiol antioxidant N-acetyl cysteine...
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