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Series of 3- and 19-oximes of 16α,17α-cyclohexanoprogesterone derivatives (pregna-d′-pentaranes) have been synthesized with the aim of probing the surfaces of progesterone receptor's and two other protein ligand binding pockets neighboring to 3- and 19-positions of steroid core. The same derivatives were also studied as possible intermediates for attachment to matrixes. The data on affinity constants...
[ 3 H]labeled progesterone and a number of its 16α,17α-cycloalkano derivatives with an additional three to six-membered D' ring were investigated for mutual competition and equilibrium binding to proteins from rat uterine cytosol. The interaction of all studied [ 3 H]ligands with proteins was characterized by comparable affinity (K d in nM region) and apparent homogeneity in...
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