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Cancer–associated proteases promote peritoneal dissemination and chemoresistance in malignant progression. In this study, kallikrein–related peptidases 4, 5, 6, and 7 (KLK4–7)–cotransfected OV–MZ–6 ovarian cancer cells were embedded in a bioengineered three–dimensional (3D) microenvironment that contains RGD motifs for integrin engagement to analyze their spheroid growth and survival after chemotreatment...
The behaviour of cells cultured within three-dimensional (3D) structures rather than onto two-dimensional (2D) culture plastic more closely reflects their in vivo responses. Consequently, 3D culture systems are becoming crucial scientific tools in cancer cell research. We used a novel 3D culture concept to assess cell–matrix interactions implicated in carcinogenesis: a synthetic hydrogel matrix equipped...
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