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A novel technique for determining the existence or nonexistence of a test nucleotide on a strand of DNA is provided. The determination advantageously uses an exonucleolytic agent that is capable of retaining a labeled nucleotide in a primer if there is a match between the test nucleotide on the strand of DNA and the complementary nucleotide on the primer, but not if there is a mismatch. The presence...
A novel technique for determining the existence or nonexistence of a test nucleotide on a strand of DNA is provided. The determination advantageously uses an exonucleolytic agent that is capable of retaining a labeled nucleotide in a primer if there is a match between the test nucleotide on the strand of DNA and the complementary nucleotide on the primer, but not if there is a mismatch. The presence...
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