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To study the effect of Butylbenzyl phthalate inducing DNA damages in Rat lung cells. KCl-SDS assay and comet assay were used to reveal the amount of DNA-protein crosslinks and effect of DNA damages induced by different doses of Butylbenzyl phathalate in vitro. The results showed that Butylbenzyl phathalate could cause DNA breakage at low level (10 μmol/L, P<;0.01), while at higher level (40 μmol/L,...
Accumulating evidences demonstrate that the channeling of nitric oxide (NO) into S-nitrothiols (SNOs) plays a pivotal role in mediating NO actions. Recent work suggests that GSNO reductase (GSNOR), an enzyme responsible for SNO metabolism, is abnormally elevated under asthmatic conditions. To study GSNOR induction in mice lung, male Kun Ming mice were exposed to gaseous formaldehyde (FA) of 0, 3.0...
The purpose of the present study was to examine levels of mRNA for DNA methyltransferases (DNMTS) and methyl- CpG-binding proteins 2 (MeCP2 ) in the lung of mouse after middle-level gaseous formaldehyde exposure. Male mice were exposed to clean air or 3.0 mg/m3 formaldehyde for consecutive 72 h and the lungs were dissected for RNA extraction. The mRNA levels of dnmtl, tin in t3a and mecp2 gene were...
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