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We developed a quantitative detection method that can reflect the degree of visceral cell oxidative DNA damage induced by exogenous compound exposures using 8-OHdG as a biomarker in vitro. In this study formaldehyde (FA) was used as a model exogenous compound and malondialdehyde (MDA) was used as a reference indicator. Rat liver cell suspension was applied in this in vitro exposure experiments as...
How does inhaled formaldehyde transfer the genotoxicity from exposure organ to site distant organs via blood? This is the key question to understand leukemia induced by formaldehyde. To answer this question, we used new born calf serum (NBS) as "blood" and Hela cells as materials to investigate its effects on cellular thiol concentration and DNA-protein crosslink (DPC) induced by formaldehyde...
The purpose of the present study was to examine levels of mRNA for DNA methyltransferases (DNMTS) and methyl- CpG-binding proteins 2 (MeCP2 ) in the lung of mouse after middle-level gaseous formaldehyde exposure. Male mice were exposed to clean air or 3.0 mg/m3 formaldehyde for consecutive 72 h and the lungs were dissected for RNA extraction. The mRNA levels of dnmtl, tin in t3a and mecp2 gene were...
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