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Objective To construct a clustered, regularly interspaced, short palindromic repeats (CRISPR)/cas9 system and use this system to obtain a recombinant Escherichia coli strain possessing the fatty acid metabolism genes from a lipid-rich marine bacterium. Results The fatty acid regulatory transcription factor (fadR), delta9 (Δ9 desaturase) and acetyl-CoA carboxylase (acc) genes were cloned from Shewanella frigidimarina...
A recombinant xylanase gene ( rxynUMB ) from Ustilagomaydis 521 was expressed in Pichia pastoris , and the enzyme was purified and characterized. Phylogenetic analysis demonstrated that rxynUMB belongs to glycosyl hydrolase family 11. The Trp84, Trp95, Glu93, and Glu189 residues are proposed to be present at the active site. The apparent molecular...
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