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A new electrogenerated chemiluminescence biosensor was fabricated by immobilizing ECL reagent Ru(bpy) 32+ and alcohol dehydrogenase in sol–gel/chitosan/poly(sodium 4-styrene sulfonate) (PSS) organically modified composite material. The component PSS was used to immobilize ECL reagent Ru(bpy) 32+ by ion-exchange, while the addition of chitosan was to prevent the cracking of...
An ethanol biosensor based on electrogenerated chemiluminescence detection was developed. Electrogenerated chemiluminescence reagent tris(2,2′-bipyridyl)ruthenium (II) and alcohol dehydrogenase were immobilized in the same sol–gel hybrid film. The copolymer poly(vinyl alcohol) with 4-vinylpyridine and cation exchanger Nafion were incorporated into sol–gel film to provide the microenvironment for retaining...
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