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Human metallothionein (hMT) is highly overexpressed in the resistant AML-2 cell line selected by paraquat, an intracellular superoxide generator. The total RNA obtained from the paraquat-resistant AML-2 cell subline was purified and reverse-transcribed into cDNA using an oligo(dT) primer. A PCR fragment for hMT was generated and cloned. Nucleotide sequence analysis revealed that the hMT transcript...
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