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The mechanisms behind reduced bacterial sensitivity to drugs and the nature of this phenomenon were explored. Escherichia coli were treated with a sub-inhibitory dose or a repeatedly doubled dose of enrofloxacin (EN) in vitro. After 10 serial passages, the minimal inhibitory concentration (MIC) of EN to the bacteria was assessed by changes in expression of genes involved in the SOS response, drug...
The effect on gene expression and cell morphology of Escherichia coli strain ATCC 25922 of exposure to the antibiotic enrofloxacin was studied. The growth rate of the cells was compromised, and the cells took on a filamentous shape. Microarray analysis showed that the expression of 519 genes was altered by the treatment, of which 239 were up- and 280 down-regulated. The transcription of 34 genes was...
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