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Gene sequence coding of a kiwi pectin methylesterase inhibitor was optimized, synthesized, and expressed in Pichia pastoris GS115 based on P. pastoris preferred codon usage. The expression level of the recombinant protein (kwPMEI) increased by 89.74% after codon optimization. Expression conditions of recombinant strains were optimized. The highest production of kwPMEI was achieved using 0.8% sorbitol...
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