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As a critical gaseous signaling molecule, H2S is involved in various biological processes. To deeper study the physiological and pathological roles of H2S, convenient and efficient detection techniques for endogenous H2S in vivo are still in urgent demand. Herein, we reported a new turn-on Near-infrared (NIR) fluorescence probe NIR-H2S based on thiolysis reactions for detection of H2S. The probe possessed...
In this study, a rapid, sensitive, and specific assay for detecting miRNAs was developed based on a two-stage amplification reaction (TSAR) using molecular beacons (MBs) as turn-on probes. In the TSAR, different miRNAs can be converted to the same reporter oligonucleotides (Y), which can hybridize with the same MB. Therefore, in combination with specific templates, this method can be applied to multiplex...
We report a novel magnetic relaxation-based sensing method for sensitive and selective detection of phosphate ions in aqueous media using paramagnetic nanoparticles. The method can detect phosphate ions at physiological pH quantitatively with high selectivity, even in a commercial fertilizer without separation.
In this study, a novel hydrophilic–lipophilic balanced magnetic nanoparticle, magnetic poly(divinylbenzene-co-N-vinylpyrrolidone) (HLB-MPNP) was successfully synthesized and applied for the extraction and determination of triazine and organochlorine pesticides in environmental water samples. The specific ratio of two monomers, hydrophilic N-vinylpyrrolidone and lipophilic divinylbenzene, endowed the...
A facile electrochemical immunosensor based on graphene–three dimensional nanostructure gold nanocomposites (G–3D Au) using simple and rapid one-step electrochemical co-reduction technique was developed for sensitive detection of topoisomerase. The resultant G–3D Au nanocomposites were characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy, and...
A rapid and sensitive fluorescence polarization immunoassay (FPIA), based on a polyclonal antibody, has been developed for the detection of sodium benzoate in spiked samples. The immunogen and fluorescein-labeled analyte conjugate were successfully synthesized, and the tracer was purified by TLC. Under the optimal assay conditions, the FPIA shows a detection range of 0.3–20.0μgmL −1 for sodium...
The inert gas fusion and infrared absorption and thermal conductivity methods are widely used for quantitative determination of oxygen(O), nitrogen(N) and hydrogen(H) in metals. However, O, N and H cannot be determined simultaneously with this method in most cases and the sensitivity cannot meet the requirement of some new metal materials. Furthermore, there is no equipment or method reported for...
A new bio-mimetic bulk acoustic wave (BAW) sensor for paracetamol, with a high selectivity and sensitivity, was fabricated by using the molecularly imprinted polymer (MIP) as the sensing material. Non-covalent molecular imprinting polymers were synthesized simultaneously using two different functional monomers, namely the weakly basic 4-vinylpyridine (4-VP) and the acidic and hydrogen binding methacrylic...
Graphite powder-supported nickel(II) hexacyanoferrate (NiHCF) was prepared by the in situ chemical deposition method and then dispersed into methyltrimethoxysilane-derived gels to form a conductive composite. The composite was used as electrode material to construct a surface-renewable three-dimensional NiHCF-modified carbon ceramic electrode. Electrochemical behavior of the chemically modified electrode...
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