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Recombinant rotavirus VP6 expressed in transgenic tomato cells was found primarily in the intracellular fraction and had a molecular weight of 44 kDa. In a shake flask, transgenic tomato cells produced 0.33 mg recombinant VP6 l−1 after 18 days of incubation. In a high aspect rotating-wall vessel designed by NASA to simulate microgravity, the transgenic cells produced up to 0.15 mg recombinant VP6...
The recombinant plasmids harboring a heterologous gene coding mouse endostatin were transfected and expressed stably in Drosophila melanogaster S2 cells. Recombinant endostatin expressed in the stably transformed S2 cells was secreted into the medium. Recombinant endostatin was also purified to homogeneity using a simple one-step Ni2+ affinity fractionation method. The purification yield was approximately...
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