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The O-polysaccharide (O-antigen) was isolated by mild acid degradation of the lipopolysaccharide (LPS) of Escherichia coli O110. The following structure of the linear tetrasaccharide O-unit of the O-polysaccharide was established by sugar analysis along with 1D and 2D 1 H and 13 C NMR spectroscopy: where aThr indicates allothreonine. The O-antigen gene cluster of E. coli O110 was sequenced...
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