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Here, we propose a simple and novel “signal-on” electrochemiluminescence (ECL) biosensor based on resonance energy transfer (RET) for detection of prostate specific antigen (PSA). The system is composed of Multi-walled carbon nanotubes (MWCNT), polyamidoamine (PAMAM) dendrimer and Au nanoparticles (NPs) film on glassy carbon electrode (GCE) to improve the electron transfer, provide abundant amine...
In the present work, two morphologies of SiO 2 nanomaterials (SiO 2 nanotubes and nanoparticles) have been successfully synthesized in supercritical fluids (SCFs). The cataluminescence (CTL) features of the two SiO 2 nanomaterials to some common harmful gases were compared, and the results showed that SiO 2 nanotubes had better CTL sensing characteristic to some common...
Thin antimony oxide covered AuSb alloy electrode was firstly found to be an excellent cold cathode for generating hot electrons during cathodic pulse polarization. Owing to the injection of hot electrons and the subsequent generation of hydrated electrons, fluorescein iso-thiocyanate (FITC) that cannot be excited in common ECL was cathodically excited at the alloy electrode. Self-assembled thiol monolayers...
A reagentless signal-on electrochemiluminescence (ECL) biosensor for DNA hybridization detection was developed based on the quenching effect of ferrocene (Fc) on intrinsic cathodic ECL at thin oxide covered glassy carbon (C/C x O 1−x ) electrodes. To construct the DNA biosensor, molecular beacon (MB) modified with ferrocene (3′-Fc) was attached to a C/C x O 1−x electrode...
A highly sensitive “two-site” chemiluminescent immunoassay specific for human thyroid stimulating hormone (TSH) was developed. The signal amplification was achieved via a biotin–streptavidin system (BSAS). The HRP–luminol–H 2 O 2 chemiluminescent system with high sensitivity was chosen as the detection system. Biotinylated anti-TSH monoclonal antibody (MAb) and HRP-labeled streptavidin...
A coulometric detector based on carbon felt as working electrode has been designed. Ascorbic acid, hydroquinone, gallic acid and sulfur dioxide were used as electroactive compounds to determine the electrochemical characteristics of this detector. The coulometric conversion efficiency, selectivity, linear response range, detection limit and mass transfer coefficient were investigated in order to use...
The electrogenerated chemiluminescent (ECL) behavior of hemin at a platinum electrode in the alkaline solution has been investigated in detail. Under the optimum conditions the linear response range of hemin is 1.0x10 -5 -1.0x10 -8 g ml -1 , the detection limit was 1.0x10 -8 g ml -1 , and the relative standard derivation for 1x10 ...
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