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The purpose of this study is to establish a simple method to incorporate materials into fertilized fish eggs by using some voltage pulses. Medaka (Oryzias latipes) eggs are used in our research. Medaka fish lay a lot of eggs every day, so it is easy to prepare samples for experiment. Furthermore we can observe their growth through transparent outer-shell with a microscope.
The purpose of this study is to investigate the effects of pulsed electric fields on fertilized eggs and to establish a technique of incorporating materials into them without damage to inside tissues, embryo-cell and yolk. Medaka (Oryzias latipes) egg was used, since its growth can be observed easily with a microscope because of its transparency of outer-shell. In addition, it is easy to handle and...
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