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S‐adenosyl‐l‐methionine (SAM) is a highly valued chemical that can be used as a dietary supplement and has been used to treat depression, osteoarthritis, and liver problems as well. We adopted systems metabolic engineering strategies to improve SAM production in a high‐producing strain (GS115/DS56). First, the cystathionine β‐synthase gene CYS4 was downregulated using a weak promoter PG12 to reduce...
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