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A permanent epithelial‐like cell line, named GrB, was established from brain tissues of the rare minnow (Gobiocypris rarus). GrB cells grow well at temperatures between 22℃ and 27℃ in L‐15 medium supplemented with 10% FBS. Chromosome analysis revealed that GrB cells possessed a diploid chromosome number of 2n = 50 at passage 50, consistent with the number of chromosomes reported for G. rarus. Partial...