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In this work, a sensitive, universal and reusable electrochemical biosensor based on stannic oxide nanocorals-graphene hybrids (SnO2 NCs-Gr) is developed for target DNA detection by using two kinds of DNA enzymes for signal amplification through an autonomous cascade DNA duplication strategy. A hairpin probe is designed composing of a projecting part at the 3′-end as identification sequence for target,...
In the current study, zirconia (ZrO 2 ) and its composite, magnesia–zirconia (MgO–ZrO 2 ), were prepared as the hydrophilic interaction chromatographic (HILIC) stationary phases (SPs). Different experimental variables including water content, pH and buffer concentration in the mobile phase (MP) as well as column temperature were systematically studied to permit an in-depth understanding...
A novel method that combines two concentration techniques, off-line centrifuge microextraction (CME) and on-line sweeping, is used to determine trace steroids in urine by micellar electrokinetic chromatography (MEKC). The CME–sweeping–MEKC is a promising technique, which has a variety of merits such as high sensitivity, rapid operation, minimal cost, and high sample throughput capability. Using CME–sweeping,...
Though liquid chromatography electrospray tandem mass spectrometry (LC–ESI-MS 2 ) has been widely used in the structural elucidation of triacylglycerols (TAG) in vegetable oils, its potentiality for the identification of TAG molecules in omega-3 rich oils remains unexplored till date. Hence, this article investigates the applicability of LC–ESI-MS 2 for the structural characterization...
A solid-state electrochemiluminescence (ECL) biosensing switch based on special ferrocene-labeled molecular beacon (Fc-MB) has been successfully developed for T4 DNA ligase detection. Such special switch system consisted of two main parts, an ECL substrate and an ECL intensity switch. The ECL substrate was made by modifying the complex of Au nanoparticle and Ruthenium (II) tris-(bipyridine) (Ru(bpy)...
In this protocol, a fluorescent aptasensor based on magnetic separation for simultaneous detection thrombin and lysozyme was proposed. Firstly, one of the anti-thrombin aptamer and the anti-lysozyme aptamer were individually immobilized onto magnetic nanoparticles, acting as the protein captor. The other anti-thrombin aptamer was labeled with rhodamine B and the anti-lysozyme aptamer was labeled with...
A simple, sensitive, and accurate method for determination of polybrominated diphenyl ethers (PBDEs) in soil has been developed based on headspace solid-phase microextraction (HS-SPME) followed by gas chromatography–mass spectrometry (GC–MS). Permethylated-β-cyclodextrin/hydroxyl-termination silicone oil (PM-β-CD/OH-TSO) fiber was first prepared by sol–gel technology and employed in SPME procedure...
Ultrasonic solvent extraction combined with solid-phase microextraction (SPME) with calix[4]arene/hydroxy-terminated silicone (C[4]/OH-TSO) oil coated fiber was used to extract phthalate acid esters (PAEs) plasticizers in plastic, such as blood bags, transfusion tubing, food packaging bag, and mineral water bottle for analysis by gas chromatography (GC). Both extraction parameters (i.e. extraction...
A sensitive method for the determination of lead in preserved egg by flame absorption spectrometry using ammonium pyrrolidine dithiocarbamate-polystyrene chemically modified platinum wire matrix is presented. The modified platinum wire matrix, after preconcentrating the lead, is placed in a flame burner for direct atomization and measurement. The concentration range is linear between 5 and 500 ng/ml...
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