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Heating at 100°C for 5–10min is a common method for treating wastewater containing recombinant DNA in many bio-laboratories in China. In this experiment, plasmid pET-28b was used to investigate decay efficiency of waste recombinant DNA during thermo-treatment. The results showed that the decay half-life of the plasmid was 2.7–4.0min during the thermo-treatment, and even heating for 30min the plasmids...
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