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Hepatitis C virus non-structural protein NS5A plays an important role in viral replication and various cellular events. To gain further insight into the function of NS5A, we screened a human fetal liver cDNA library for its interacting proteins using the yeast two-hybrid system. FKBP38, a 38kDa immunosuppressant FK506-binding protein, was identified and its interaction with NS5A was confirmed by both...
It has been suggested that cellular proteins are involved in hepatitis C virus (HCV) RNA replication. By using the yeast two-hybrid system, we isolated seven cDNA clones encoding proteins interacting with HCV RNA polymerase (NS5B) from a human liver cDNA library. For one of these, α-actinin, we confirmed the interaction by coimmunoprecipitation, immunofluorescent staining and confocal microscopic...
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