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A flow-through quartz crystal microbalance (QCM) immunoassay method has been developed based on aflatoxin B1 antibody (anti-AFB1)-functionalized magnetic core-shell Fe3O4/SiO2 composite nanoparticles (bionanoparticles) in this study. To construct such an assay protocol, anti-AFB1, as a model protein, was initially covalently immobilized onto the Fe3O4/SiO2 surface, and then the functionalized nanoparticles...
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