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A specific multiplex polymerase chain reaction (PCR) was applied to differentiate samples of razor clams Ensis arcuatus, Ensis siliqua, Ensis directus, and Ensis macha. Universal primers were used for the amplification of internal transcribed spacer 1 (ITS-1) in each species. The alignment of the obtained sequences was the basis for the specific design of species-specific reverse primers (ITSArSil-R,...
Ensis arcuatus and Ensis siliqua are the most economically important species of razor clams in the European Union. Due to similarities between their shell morphology, and the differing retail value, these species are often misidentified. Therefore, it is necessary to develop an appropriate protocol to allow accurate differentiation between these species of razor clam in order to protect consumer...
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