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Papain is a cysteine protease with wide substrate specificity and many applications. Despite its widespread applications, cold stability of papain has never been studied. Here, we used differential spectroscopy to monitor thermal denaturation process. Papain was the most stabile from 45°C to 60°C with ΔG°321 of 13.9±0.3kJ/mol and Tm value of 84±1°C. After cold storage, papain lost parts of its native...
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