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A fusion protein of single-chain, Fv-tumor necrosis factor α, scFv-TNF, was constructed and expressed in the Pichia pastoris expression system. There was 67-fold less toxicity of the fusion protein when compared with TNF-α alone in cells expressing CA125. Furthermore, scFv-TNF was 10-fold more selective in cell killing directed by anti-CA125 scFv in a CA125-positive cell line than the CA125-negative...
Fusion protein of single-chain Fv-tuftsin (scFv-tuftsin) was constructed and expressed in yeast Pichia pastoris expression system. Immunisation of mice with scFv-tuftsin fusion protein enhanced the humoral immune responses, including anti-idiotypic (Ab2) and anti-anti-idiotypic (Ab3) inductions, when compared to scFv alone as a control. In marked contrast to response pattern in Ab2 induction, there...
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