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All the known members of the mouse Notch receptor family were examined for their biochemical function by interaction with a DNA binding protein RBP-Jκ. mNotch2, mNotch3 and int3 (=mNotch4) were shown to interact with RBP-Jκ by the GST-fusion pull down assay and dominant negative competition with Epstein Barr virus nuclear antigen 2. Furthermore the intracellular region of int3 was shown to transactivate...
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