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Zwei kurze Sonden auf Basis „fixierter“ („locked“) Nucleinsäuren (LNAs) können microRNAs durch simple Hybridisierung bei zwei Temperaturen abfangen und detektieren. So lassen sich intakte microRNAs hoch spezifisch und mit einem Dynamikbereich von fast vier Größenordnungen direkt bis hinunter zu attomolaren Konzentrationen bestimmen. Einzelbasenfehlpaarungen in den microRNAs werden effizient von den...
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