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We have used single cell fluorescence imaging techniques to examine how functional properties of the caffeine-sensitive Ca 2+ store change during differentiation of a sub-population of caffeine-sensitive SH-SY5Y cells. Application of caffeine (30mM) 1–10.5min after a ‘priming’ depolarisation pulse of 55mM K + revealed that the caffeine-sensitive store in undifferentiated cells remained...
We have used single cell fluorescence imaging techniques to examine the role that ryanodine receptors play in the stimulus-induced Ca 2+ responses of SH-SY5Y cells. The muscarinic agonist methacholine (1mM) resulted in a Ca 2+ signal in 95% of all cells. Caffeine (30mM) however stimulated a Ca 2+ signal in only 1–7% of N-type (neuroblastic) cells within any given field. The...
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