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To improve the enantioconvergence of an epoxide hydrolase from Phaseolus vulgaris (PvEH1) towards styrene epoxides, its directed modification was performed based on the rational design by using the molecule docking simulation and the multiple alignment. The single- and multi-site mutation genes of pveh1 were constructed as designed theoretically by PCR, and expressed in E. coli BL21(DE3), respectively...
Ammonium chloride is a very inexpensive and readily available reagent, and efficiently catalyzes the thia-Michael addition reaction of thiols to different α-enone Michael acceptors in water. Reasonable to good yields of the desired products were obtained in most cases.
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