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Objective and Method
We developed and evaluated five novel real‐time RT‐PCR assays targeting conserved regions in the 5′‐untranslated region (5′‐UTR), envelope (E'), non‐structural protein 2A (NS2A), NS5 and 3′‐UTR of the ZIKV genome.
Results
The ZIKV‐5′‐UTR assay exhibited the lowest in vitro limit of detection (5–10 RNA copies/reaction and 3.0 × 10−1 plaque‐forming units/ml). Compared to the...
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