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PEGylation, used to mitigate some of the problems that affect the effectiveness of therapeutic proteins, often results in a heterogeneous population of conjugated species and unmodified protein that presents a protein separations challenge. This study presents the use of a mildly amphiphilic support, Tris-capped CH Sepharose 4B as an alternative for separating PEGylated proteins from their unmodified...
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