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Zona pellucida (ZP) proteins are glycoproteins in fish chorion that are encoded by multiple gene families, mainly including zp1, zp2, and zp3. In the present study, we cloned two zp genes of half-smooth tongue sole, Cynoglossus semilaevis, using reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The two genes were demonstrated to belong to zp3 isoforms...
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