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According to the purification characteristics of lipase from Burkholderia ambifaria YCJ01, mesoporous TiO2 was functionalized by phenylaminopropyl trimethoxysilane (Ph-TiO2) for lipase immobilization. This support permitted one step immobilization and purification of lipase YCJ01 via hydrophobic interactions from crude fermentation supernatant. The activity of immobilized lipase was 6119.2U/g support...
Alcohol dehydrogenases can catalyze the inter-conversion of aldehydes and alcohols. The t-butyl 6-chloro-(3R,5S)-dihydroxyhexanoate is a key chiral intermediate in the synthesis of statin-type drugs such as Crestor (rosuvastatin calcium) and Lipitor (atorvastatin). Herein, a novel alcohol dehydrogenase (named as KleADH) discovered from Klebsiella oxytoca by a genome mining method was cloned and characterized...
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