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PHB biosynthesis pathway, consisting of three open reading frames (ORFs) that encode for β-ketothiolase (phaACma, 1179 bp), acetoacetyl-CoA reductase (phaBCma, 738 bp), and PHA synthase (phaCCma, 1694 bp), of Caldimonas manganoxidans was identified. The functions of PhaA, PhaB, and PhaC were demonstrated by successfully reconstructing PHB biosynthesis pathway of C. manganoxidans in Escherichia coli...
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