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One of the critical factors restricting the progress in crop breeding and genetic studies is the time required in generating desired ‘pure line’ segregating populations. We developed a procedure that can advance up to seven generations of canola per annum, allowing the production of pure line populations within a year. An ‘all soil’ method was compared with an ‘embryo culture plus soil’ procedure...
Generating segregating populations and ‘pure lines’ is an essential component in many projects of plant breeding and biological studies. Time taken to obtain these materials is often a critical factor restricting the progress of such projects. We report a procedure here which, by combining embryo culture with managements of watering regimes, lighting intensity and duration, temperature and quantity...
Compared with hexaploid wheat, tetraploid durum is more susceptible to Fusarium crown rot (FCR) infection. The feasibility of enhancing FCR resistance in durum wheat by introgressing chromosome segments of hexaploid wheat was investigated by generating and analysing a backcross population derived from a susceptible durum wheat variety ‘Bellaroi’ (recurrent parent) and a resistant hexaploid genotype...
By essentially fixing the genetic background, near-isogenic lines (NILs) are ideal for studies of the function of specific loci. We report in this paper the development of NILs for a major QTL located on the long arm of chromosome 3B conferring Fusarium crown rot (FCR) resistance in hexaploid wheat. These NILs were generated based on the method of the heterogeneous inbred family analysis. 13 heterozygous...
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