The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
The article is about induction of apoptosis in human hepatoma HepG2 cells by myricetin (MYR). The human hepatoma HepG2 cells was treated with different doses myricetin for 72 h. Cytotoxicity was evaluated by SRB assay, apoptosis-related alterations in morphology ascertained by cytochemical staining(Hoechst 33258) and transmission electron microscopy (TEM). The cell cycle and apoptotic rate were quantified...
Currently, the research on indoor air pollution had become one of the hot subjects in the field of environment science. Toluene diisocyanate was a kind of indoor air pollutant with low toxic, but the most toxic in all diisocyanate esters, which was recognized as a kind of industry allergen. It was studied in this issue that the effect of toluene diisocyanate ocontent on superoxide dismutase, malondialdehyde...
To study the effect of qinglongyi polysaccharide on cation concentration in erythrocyte of S180 mice a tumor mouse model was developed, and the mice were treated with high, medium, and low doses of qinglongyi polysaccharide through abdominal injection for 7 d. Erythrocytes were collected and erythrocyte suspensions were prepared. Change of Ca2+ concentration in the erythrocytes of S180 mice was measured...
To study the effect of Saponins of asparagus on HepG2 apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential (Deltapsim) levels. Saponins of asparagus with different concentration was treated with HepG2 at different time, MTT assay was used to detect inhibitory rate, fluorescence staining was used to observe apoptosis morphology, flow cytometry was used to detect apoptosis rate...
The newly-developed blood immunological reaction pathway diagram experiment system has been used in a series of studies on the role of erythrocytes in regulating the immunological reactions of lymphocytes, and it has been found that when the tumor antigen S180 is activated, erythrocytes can increase the expression of CD25 on the surfaces of the surrounding hemolymphocytes, which indicates the immunological...
The aim of this paper is to investigate the pro-apoptosis effect of isothiocyanates (ITCS) on human gastric adenoma cells SGC-7901 and its mechanism. SGC-7901 was treated with different concentrations of ITCS. MTT assay was used to evaluate the influence of ITCS on cell proliferation. Flow cytometry was used to test reactive oxygen species (ROS) levels, intracellular mitochondrial transmembrane potential...
To study the effect of growth inhibition and induction of apoptosis in sarcoma 180 cells by juglone in vivo. S180 tumor-bearing mice were set up to investigate the effects of juglone on tumor growth inhibition and apoptosis. After HE staining, the pathomorphologic changes of tumor cells were observed by optical microscope. The ultrastructural changes of tumor cells were observed by transmission electron...
To explore how to calculate the effect of solanine on the Michaelis constant and the maximum reaction rate of NAT, high performance liquid chromatography (HPLC) was used, with 2-AF as substrate, and the rate at which 2-AF is acetylated into 2-AAF in intact HepG2 cells or in the cytoplasm of HepG2 cells as the reaction rate. The double reciprocal plot was made, with 1/S (reciprocal of the concentration...
To investigate the pro-apoptosis effect of isothiocyanates(ITCS) on human gastric adenoma cells SGC- 7901 and its mechanism. METHODS: SGC-7901 was treated with different concentrations of ITCS. MTT assay was used to evaluate the influence of ITCS on cell proliferation. Flow cytometry was used to test reactive oxygen species (ROS) levels, intracellular mitochondrial transmembrane potential (Deltapsim),...
To observe the effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca24]; in the cells, and to uncover the mechanism by which solanine induces apoptosis. HepG2 cells are double stained with TMRE and Fluo- 3/AM, and both the change in membrane potential of mitochondria and that of [Ca2+]; in the cells are observed using LCSM.The results of double staining with TMRE and...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.