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Bacterial contamination of in vitro vs in vivo produced embryos presents a particular danger because of the alteration of the zona pellucida and the use of various biological products during culture. Our objective was to investigate the effects of semen contaminated with bacteria on IVF of bovine oocytes and to determine if removal of cumulus cells by vortexing as opposed to pipetting would reduce...
The objective of this study was to investigate the effect of serum on in vitro maturation of Korean Native Cattle(KNC) oocytes. Oocytes were collected by slicing the ovaries of slaughtered Korean Native Cows with a surgical blade and matured in TCM199 supplemented with 10% FBS, 5%, 10%, and 20% steer serum(SS), 5%, 10% and 20% porcine serum(PS), 5%, 10% and 20% chicken serum(CS) in 5% CO 2 ...
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