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We investigated the contribution of apoE to cholesterol efflux into plasmas of normal, apoA-I-, and apoE-deficient mice. Plasmas of normal and apoA-I-deficient mice contain apoE in preβ-migrating VLDL as well as in HDL-like lipoproteins which have either electrophoretic α- or γ-mobilities. The latter particle resembled to γ-LpE in human plasma also by its mobility upon non-denaturing two-dimensional...
In the reverse cholesterol transport HDL with press 1 -mobility are the initial acceptors of cell membrane cholesterol. To study the origin of press 1 -HDL plasmas were incubated for 16h at +37°C in the presence and absence of phospholipid transfer protein (PLTP). Distribution of HDL-particles was analyzed by non-denaturing two-dimensional electrophoresis and anti-apoA-I immunoblotting...
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