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Effects of sulfhydryl modification on the ATP regulated K 1 channel (K ATP channel) in the pancreatic β-cell were studied, using the patch clamp technique. Application of the sulfhydryl oxidizing agents thimerosal and 2,2'-dithio-bis(5-nitropyridine) (DTBNP), in micromolar concentrations, caused complete inhibition of the k ATP channel, in inside-out patches...
The sulphydryl reagent thimerosal (50 μM) released Ca 2+ from a non-mitochondrial intracellular Ca 2+ pool in a dose-dependent manner in permeabilized insulin-secreting RINm5F cells. This release was reversed after addition of the reducing agent dithiothreitol, Ca 2+ was released from an Ins(1,4,5)P 3 -insensitive pool, since release was observed even...
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