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A scaled-up SRCCC apparatus equipped with a 40-L column was constructed on the basis of separations of crude broccoli seed extract and crude radish seed extract using a conventional SRCCC instrument. Scaled-up separation of 500 g of crude broccoli seed extract with n -butanol-acetonitrile-10% (NH 4 ) 2 SO 4 aqueous solution 1:0.5:2 (υ/υ) as solvent system yielded 61.5 g glucoraphanin product of purity...
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