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Transcription of genes occurs in apparently irregular pulses of activity termed transcriptional bursts. In contrast, cellular differentiation is marked by coordinated gene expression of batteries of genes required for cellular specialization. Ocular lens is comprised of two compartments: the lens epithelium and lens fibers. Position of individual cells within the lens is directly related to their...
Purpose
Embryonic lens development requires intricate temporal and spatial control of gene expression that is executed through specific gene regulatory networks regulated by FGF signaling. The αA‐crystallin is the most abundant mammalian lens protein; transcriptional studies of this gene provide detailed insights into the molecular mechanisms governing lens fiber cell differentiation.
Methods
Dynamic...
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