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We describe the unusual case o f early-onset adenocarcinoma o f the cardia in a 22year-old male. The patient died within less than one year after diagnosis was established. By immunohistochemistry, pS3 expression was observed in the tumor cells. Automated direct sequencing o f polymerase chain reaction (PCR) amplified DIVA revealed a homozygous transition in pS3 codon 280 (AGA to G GA) as the molecular...
Zusammenfassung. Hypervariable Regionen (HVR) als hochpolymorphe DNA-Sequenzen knnen als Marker in der molekular-genetischen Diagnostik in verschiedenen Gebieten der Medizin benutzt werden. Wir beschreiben die methodischen Grundlagen dieser Technik, die auf der Polymerasekettenreaktion (PCR) und der anschlieenden elektrophoretischen Auftrennung der HVR-Amplifikationsprodukte basieren. An 2 Fllen wird...
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