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Enzymatic analyte recycling results in detection limits at the nanomolar level thus allowing for the coupling to immunoassay detection. For this reason a bifunctional reagent was synthesized, which has the two properties of being a redox active substance, for a bi-enzymatic redox-cycling system on one hand, and as being a competitor, e.g. a label, for the analyte in an immunoassay on the other hand...
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