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The sensing performance of DNAzymes in live cells is tremendously hampered by the inefficient and inhomogeneous delivery of DNAzyme probes and their incontrollable off‐site activation, originating from their susceptibility to nuclease digestion. This requires the development of a more compact and robust DNAzyme‐delivering system with site‐specific DNAzyme activation property. Herein, a highly compact...
DNA amplification machines show great promise for intracellular imaging, yet are always constrained by off‐site machinery activation or signal leakage, originating from the inherent thermodynamically driven hybridization between machinery substrates. Herein, an entropy‐driven catalytic DNA amplification machine is integrated with the on‐site amplified substrate exposure procedure to realize the high‐contrast...
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