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Objective To construct recombinant Apoptin gene (vp3) retrovirus pLVP3 and to study its apoptosis inducing effect on human breast cancer cells 435 as well as to discuss its mechanism in vitro and in vivo. Methods vp3 gene was cloned and recombinated into retrovirus vector pLP-LNCX-VP3 (pLVP3) at loxP site, which was transformed into package cell line PT67 and then into NIH3T3 cells for titer assay...
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