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A column-switching system, composed of supercritical fluid chromatography (SFC) and reverse phase liquid chromatography/mass spectrometry (RPLC/MS) was developed for the analysis of vitamin D in oily and fatty matrices. The SFC with the similar retention behavior as normal-phase liquid chromatography (NPLC), was applied for an on-line clean-up of oily and fatty samples, then followed by separation...
Gold nanoparticles (AuNPs)-embedded paper was coupled with ion-transmission mass spectrometry (MS) to enable the highly sensitive detection of chemical warfare agent (CWA) simulants in solutions. With the assistance of a low-temperature plasma (LTP) probe, we found that AuNPs were capable to enhance the ionization efficiencies of target analytes, with MS signal intensities surprisingly undergone an...
A label-free electrochemiluminescence (ECL) cytosensor was developed for dynamically evaluating of epidermal growth factor receptor (EGFR) expression on MCF-7 cancer cells based on the specific recognition of epidermal growth factor (EGF) with its receptor (EGFR). EGF-cytosensor was fabricated by in-situ electro-polymerization of polyaniline as substrate, using CdS quantum dots (CdS QDs) as ECL probe...
DNA 3′-phosphatase takes an important role in DNA damage repair, replication and recombination. Here, we present a novel label-free fluorescent assay for T4 polynucleotide kinase/phosphatase (T4 PNKP) activity and its inhibitor screening by using poly(thymine)-templated fluorescent copper nanoparticles (CuNPs) as a fluorescent indicator. In this assay, we designed a simple T-rich hairpin primer with...
In this study, a simple and sensitive aptamer-based fluorescence method for the detection of Kanamycin A by using gold nanoparticles (AuNPs) has been developed. In this assay, AuNPs were utilized as DNA nanocarrier as well as efficient fluorescence quencher. In the absence of Kanamycin A, dye-labeled aptamer could be adsorbed onto the surface of AuNPs and the fluorescence signal was quenched. In the...
By using a visible-light-excited ternary Eu 3+ complex, BHHBCB-Eu 3+ -BPT (BHHBCB: 1,2-bis[4′-(1“,1“,1“,2“,2“,3“,3“-heptafluoro-4″,6″-hexanedion-6″-yl)-benzyl]-4-chlorosulfobenzene; BPT: 2-(N,N-diethylanilin-4-yl)-4,6-bis(pyrazol-1-yl)-1,3,5-triazine), as a luminophore, two kinds of novel visible-light-excited europium materials, the silica-encapsulated BHHBCB-Eu 3+ -BPT (Eu@SiO...
A united method for speciation analysis of Se (IV) and Se (VI) in environmental water samples was developed using nano-sized TiO 2 colloid as adsorbent and hydride generation atomic fluorescence spectrometry (HG-AFS) as determination means. When the pH values of bulk solution were between 6.0 and 7.0, successful adsorption onto 1mL nano-sized TiO 2 colloid (0.2%) was achieved for more...
The core-shell structured luminomagnetic microsphere composed of a Fe 3 O 4 magnetic core and a continuous SiO 2 nanoshell doped with Eu(DBM) 3 ·2H 2 O fluorescent molecules was fabricated by a modified Stöber method combined with a layer-by-layer assembly technique. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field emission scanning...
The electrogenerated chemiluminescent (ECL) behavior of hemin at a platinum electrode in the alkaline solution has been investigated in detail. Under the optimum conditions the linear response range of hemin is 1.0x10 -5 -1.0x10 -8 g ml -1 , the detection limit was 1.0x10 -8 g ml -1 , and the relative standard derivation for 1x10 ...
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