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S-nitrosylation is a nitric oxide (NO)-based post-translational modification regulating protein function and signalling. We used a combination between the biotin switch method and labelling with isotope-coded affinity tag to identify endogenously S-nitrosylated peptides in Arabidopsis thaliana proteins extracted from plantlets. The relative level of S-nitrosylation in the identified peptides was compared...
Tobacco cell suspension cultures respond to treatment with phorbol 12-myristate 13-acetate (PMA), by the accumulation of transcripts for hsr203J, a plant defence gene. We provide evidence that the effect of PMA on hsr203J gene expression depends upon a transient production of active oxygen species (AOS), an early response of the cell suspensions to PMA. Pre-treatment with diethyldithiocarbamate (DDC),...
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